This information is provided free of charge by the Department of Industrial Relations from its web site at These regulations are for the convenience of the user and no representation or warranty is made that the information is current or accurate. See full disclaimer at

Subchapter 7. General Industry Safety Orders
Group 16. Control of Hazardous Substances
Article 107. Dusts, Fumes, Mists, Vapors and Gases

Return to index
New query

§5154.2. Ventilation Requirements for Biological Safety Cabinets, Appendix A - High Efficiency Particulate Air Filter Aerosol Test Protocol (Non-Mandatory)

(a) Materials and apparatus

(1) Aerosol generator producing a non-hazardous oil aerosol (generated either hot or cold) of particles with a mass median diameter of less than 0.8 micrometers.

(2) Oil suitable for use in the aerosol generator when used in accordance with the generator manufacturer's instructions.

Note 1. Suitable oil has the following properties:

white mineral oil with density of 0.869 kg/L at 15 °C;

pour point -25 °C;

flash point, closed cup 144 °C;

kinematic viscosity 14.3 centistokes at 40 °C and 3.10 centistokes at 100 °C with 99% unsulphonated residue.

Note 2. Dioctylphthalate (DOP) may be used in controlled conditions where operator exposure is limited and complies with the requirements of Section 5155.

(3) Photometer having a threshold sensitivity specific to the test aerosol of 0.0001 µg/L to 120 µg/L.

(b) Procedure

(1) Operate the cabinet and introduce the aerosol challenge to the clean side of the filter and seals being tested.

Ensure that the volume of aerosol produced is sufficient to give a challenge concentration of between 50 µg/L and 100 µg/L when the area of the filter under test and the manufacturers' volumetric flow rate are accounted for. Ensure that the challenge is uniformly distributed over the filter face.

(2) Adjust the photometer to give a reading of 100 X when sampling this concentration.

(3) Using a sampling probe attached to the photometer, scan all filter faces, seals and construction joints with the probe held within 25mm of the surface and move at not more than 30 mm/s.

NOTE: Authority cited: Section 142.3, Labor Code. Reference: Section 142.3, Labor Code.


1. New Appendix A filed 10-11-94; operative 11-10-94 (Register 94, No. 41).

Go BackGo Back to Article 107 Table of Contents